 |
One of the greatest impediments to the study of toxigenic fusaria has
been incorrect and confused application of species names to Fusarium
isolates. To aid in this, we have created FUSARIUM-SEQ v.1.0, a
publicly available sequence database of partial translation elongation
factor 1-alpha (TEF) sequences, representing much of the known
diversity of the genus.
Users can generate sequences using primers that
are conserved across the genus, and use the sequence as a query to
BLAST the database, or in a phylogenetic analysis. Sequences are now
available for most known Fusarium species, with excellent
representation of the Gibberella fujikuroi species complex, Type-B
trichothecene producers, Fusarium oxysporum and F. solani species
complexes, and correct identification to a known species in these
groups often can be performed using this gene region alone.
This
growing database will contain only vouchered sequences attached to
publicly available cultures.
|